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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/11/2022 |
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Data da última atualização: |
14/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F.; HARAKAVA, R.; NOME, C F.; KITAJIMA, E. W.; ASTUA, J. de F. |
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Afiliação: |
PEDRO L. RAMOS-GONZÁLEZ, Instituto Biológico de São Paulo; CAMILA CHABI-JESUS, Instituto Biológico de São Paulo; ALINE D. TASSI, Instituto Biológico de São Paulo; RENATA FAIER CALEGARIO, Universidade Federal do Paraná; RICARDO HARAKAVA, Instituto Biológico de São Paulo; CLAUDIA F. NOME, Instituto Nacional de Tecnología Agropecuaria; ELLIOT W. KITAJIMA, Escola Superior de Agricultura Luiz de Queiroz; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Microbiology, v.28, n.13, 836076, March 2022. |
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DOI: |
https://doi.org/10.3389/fmicb.2022.836076 |
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Idioma: |
Francês |
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Conteúdo: |
An increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus (LigCSV), and Ligustrum leprosis virus (LigLV) have five open reading frames (ORFs) > 500 nts, two distributed in RNA1 and three in RNA2. RNA1 of these three viruses display the same genomic organization found in RNA1 of typical cileviruses, while their RNA2 are shorter, possessing only orthologs of genes p61, p32, and p24. LigCSV and LigLV are more closely related to each other than to SvRSV, but the identities between their genomic RNAs were lower than 70%. In gene-by-gene comparisons, ORFs from LigCSV and LigLV had the highest sequence identity values (nt sequences: 70?76% and deduced amino acid sequences: 74?83%). The next higher identity values were with ORFs from typical cileviruses, with values below 66%. Virions of LigLV (? 40 nm × 55 nm) and LigCSV (? 54 nm × 66 nm) appear almost spherical, contrasting with the bacilliform shape of SvRSV virions (? 47 nm × 101 nm). Mites collected from the virus-infected plants were identified as Brevipalpus papayensis, B. tucuman, and B. obovatus. Viruliferous B. papayensis mites successfully transmitted LigCSV to Arabidopsis thaliana. SvRSV, LigCSV, and LigLV seem to represent novel sub-lineages of kitaviruses that descent on parallel evolutionary branches from a common ancestor shared with the tentative cile-like virus hibiscus yellow blotch virus and typical cileviruses. Biological and molecular data, notably, the phylogenetic reconstruction based on the RdRp proteins in which strong support for monophyly of the family Kitaviridae is observed, mark an advance in the understanding of kitavirids. MenosAn increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus (LigCSV), and Ligustrum leprosis virus (LigLV) have five open reading frames (ORFs) > 500 nts, two distributed in RNA1 and three in RNA2. RNA1 of these three viruses display the same genomic organization found in RNA1 of typical cileviruses, while their RNA2 are shorter, possessing only orthologs of genes p61, p32, and p24. LigCSV and LigLV are more closely related to each other than to SvRSV, but the identities between their genomic RNAs were lower than 70%. In gene-by-gene comparisons, ORFs from LigCSV and LigLV had the highest sequence identity values (nt sequences: 70?76% and deduced amino acid sequences: 74?83%). The next higher identity values were with ORFs from typical cileviruses, with values below 66%. Virions of LigLV (?... Mostrar Tudo |
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Thesagro: |
Planta Ornamental; Vírus. |
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Thesaurus Nal: |
Brevipalpus; Ornamental plants; Virion. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148258/1/fmicb-13-836076.pdf
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Marc: |
LEADER 03166naa a2200277 a 4500
001 2148258
005 2022-11-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmicb.2022.836076$2DOI
100 1 $aRAMOS-GONZÁLEZ, P. L.
245 $aA Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae.$h[electronic resource]
260 $c2022
520 $aAn increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus (LigCSV), and Ligustrum leprosis virus (LigLV) have five open reading frames (ORFs) > 500 nts, two distributed in RNA1 and three in RNA2. RNA1 of these three viruses display the same genomic organization found in RNA1 of typical cileviruses, while their RNA2 are shorter, possessing only orthologs of genes p61, p32, and p24. LigCSV and LigLV are more closely related to each other than to SvRSV, but the identities between their genomic RNAs were lower than 70%. In gene-by-gene comparisons, ORFs from LigCSV and LigLV had the highest sequence identity values (nt sequences: 70?76% and deduced amino acid sequences: 74?83%). The next higher identity values were with ORFs from typical cileviruses, with values below 66%. Virions of LigLV (? 40 nm × 55 nm) and LigCSV (? 54 nm × 66 nm) appear almost spherical, contrasting with the bacilliform shape of SvRSV virions (? 47 nm × 101 nm). Mites collected from the virus-infected plants were identified as Brevipalpus papayensis, B. tucuman, and B. obovatus. Viruliferous B. papayensis mites successfully transmitted LigCSV to Arabidopsis thaliana. SvRSV, LigCSV, and LigLV seem to represent novel sub-lineages of kitaviruses that descent on parallel evolutionary branches from a common ancestor shared with the tentative cile-like virus hibiscus yellow blotch virus and typical cileviruses. Biological and molecular data, notably, the phylogenetic reconstruction based on the RdRp proteins in which strong support for monophyly of the family Kitaviridae is observed, mark an advance in the understanding of kitavirids.
650 $aBrevipalpus
650 $aOrnamental plants
650 $aVirion
650 $aPlanta Ornamental
650 $aVírus
700 1 $aCHABI-JESUS, C.
700 1 $aTASSI, A. D.
700 1 $aCALEGARIO, R. F.
700 1 $aHARAKAVA, R.
700 1 $aNOME, C F.
700 1 $aKITAJIMA, E. W.
700 1 $aASTUA, J. de F.
773 $tFrontiers in Microbiology$gv.28, n.13, 836076, March 2022.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
04/08/2022 |
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Data da última atualização: |
04/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
LOPES, L. B.; KAMCHEN, S. G.; GOMES, F. J.; NATIVIDADE, U. A.; MAGALHÃES, L. M. D.; PIMENTA, A. de P.; ARAUJO, R. N. |
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Afiliação: |
LUCIANO BASTOS LOPES, CPAMT; SCHEILA GEIELE KAMCHEN, UFMT, Sinop-MT; FAGNER JÚNIOR GOMES, USP-ESALQ, Piracicaba-SP; ULISSES ANTÔNIO NATIVIDADE, UFMG, Belo Horizonte-MG; LUISA MOURÃO DIAS MAGALHÃES, UFMG, Belo Horizonte-MG; ANGELITA DE PAULA PIMENTA, UFMG, Belo Horizonte-MG; RICARDO NASCIMENTO ARAUJO, UFMG, Belo Horizonte-MG. |
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Título: |
Influence of silvopastoral systems on gastrointestinal nematode infection and immune response of Nellore heifers under tropical conditions. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 309, 109765, 2022. |
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ISSN: |
0304-4017 |
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DOI: |
https://doi.org/10.1016/j.vetpar.2022.109765 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Among the strategies for integrating crops, livestock, and forestry, silvopastoral systems must be highlighted due to their inherent microclimatic conditions, mainly in tropical countries such as Brazil, where cattle are frequently subjected to unfavorable thermal conditions. However, according to some studies, shading can potentially worsen herds´ parasitism due to better microclimatic condition for the parasites. This study aimed to assess fecal egg count in Nellore heifers reared in two silvopastoral arrangements (pasture with single or triple tree rows), in a crop-livestock system, and open pasture. In the silvopastoral treatment composed of triple rows, lesser parasite burden means were found, with a peak infection in February/March and another in October. Regarding the effect of seasons over the year, there was an environmental influence on the egg counts, with higher averages during the late rainy season and the beginning of the dry season. An immunological investigation of animals from each group showed that cattle kept on the silvopastoral arrangements with either single or triple rows have significantly higher lymphocyte proliferation when stimulated with specific antigens than those kept on open pastures. Based on our results, it can be concluded that both silvopastoral systems were not considered as a risk factor for nematode egg counts in Nellore heifers. Indeed, the shadiest system promoted milder parasitism and higher immunological lymphocyte responses in animals. MenosAbstract: Among the strategies for integrating crops, livestock, and forestry, silvopastoral systems must be highlighted due to their inherent microclimatic conditions, mainly in tropical countries such as Brazil, where cattle are frequently subjected to unfavorable thermal conditions. However, according to some studies, shading can potentially worsen herds´ parasitism due to better microclimatic condition for the parasites. This study aimed to assess fecal egg count in Nellore heifers reared in two silvopastoral arrangements (pasture with single or triple tree rows), in a crop-livestock system, and open pasture. In the silvopastoral treatment composed of triple rows, lesser parasite burden means were found, with a peak infection in February/March and another in October. Regarding the effect of seasons over the year, there was an environmental influence on the egg counts, with higher averages during the late rainy season and the beginning of the dry season. An immunological investigation of animals from each group showed that cattle kept on the silvopastoral arrangements with either single or triple rows have significantly higher lymphocyte proliferation when stimulated with specific antigens than those kept on open pastures. Based on our results, it can be concluded that both silvopastoral systems were not considered as a risk factor for nematode egg counts in Nellore heifers. Indeed, the shadiest system promoted milder parasitism and higher immunological lymphocyte respons... Mostrar Tudo |
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Palavras-Chave: |
Sinop-MT. |
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Thesagro: |
Agrossilvicultura; Gado; Gado Nelore. |
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Thesaurus NAL: |
Eucalyptus; Fecal egg count; Helminths; Livestock; Nellore; Silvopastoral systems. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145230/1/2022-cpamt-lbl-influence-silvopastoral-systems-gastrointestinal-nematoide-infection-immune-response-nellore-heifers-under-tropical-conditions.pdf
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Marc: |
LEADER 02513naa a2200337 a 4500
001 2145230
005 2022-08-04
008 2022 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $ahttps://doi.org/10.1016/j.vetpar.2022.109765$2DOI
100 1 $aLOPES, L. B.
245 $aInfluence of silvopastoral systems on gastrointestinal nematode infection and immune response of Nellore heifers under tropical conditions.$h[electronic resource]
260 $c2022
520 $aAbstract: Among the strategies for integrating crops, livestock, and forestry, silvopastoral systems must be highlighted due to their inherent microclimatic conditions, mainly in tropical countries such as Brazil, where cattle are frequently subjected to unfavorable thermal conditions. However, according to some studies, shading can potentially worsen herds´ parasitism due to better microclimatic condition for the parasites. This study aimed to assess fecal egg count in Nellore heifers reared in two silvopastoral arrangements (pasture with single or triple tree rows), in a crop-livestock system, and open pasture. In the silvopastoral treatment composed of triple rows, lesser parasite burden means were found, with a peak infection in February/March and another in October. Regarding the effect of seasons over the year, there was an environmental influence on the egg counts, with higher averages during the late rainy season and the beginning of the dry season. An immunological investigation of animals from each group showed that cattle kept on the silvopastoral arrangements with either single or triple rows have significantly higher lymphocyte proliferation when stimulated with specific antigens than those kept on open pastures. Based on our results, it can be concluded that both silvopastoral systems were not considered as a risk factor for nematode egg counts in Nellore heifers. Indeed, the shadiest system promoted milder parasitism and higher immunological lymphocyte responses in animals.
650 $aEucalyptus
650 $aFecal egg count
650 $aHelminths
650 $aLivestock
650 $aNellore
650 $aSilvopastoral systems
650 $aAgrossilvicultura
650 $aGado
650 $aGado Nelore
653 $aSinop-MT
700 1 $aKAMCHEN, S. G.
700 1 $aGOMES, F. J.
700 1 $aNATIVIDADE, U. A.
700 1 $aMAGALHÃES, L. M. D.
700 1 $aPIMENTA, A. de P.
700 1 $aARAUJO, R. N.
773 $tVeterinary Parasitology$gv. 309, 109765, 2022.
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Embrapa Agrossilvipastoril (CPAMT) |
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